6. 7/8 Future prospects: there was so little biochemical data for our specific proteins, we had to rely on genome-wide data (PBMs). We could then estimate Kd for ANY sequence. That means, also for sequence variants.

Our earlier Framework Model v2 (FMv2) and FM-Life models link the clock right up, through plant metabolism, growth, development and physiology, to seed-to-seed adaptation in real weather.

Anyone who assembles these parts gets to predict how an Arabidopsis genome sequence variant will be adapted to climatic conditions. With a full causal chain. The mechanisms of evolutionary adaptation. #GoForIt

Sure it’s simplified and there’s much to validate but it’s there. DNA-binding Kd is just where we started, there’s more known biochemistry to add.

5/8 The simulated Kd values for the Evening Complex binding overlapped with the empirical data on its DNA-binding protein LUX

Simulated CCA1/LHY bound much less tightly than its measured Kd. Likely, the plant regulates these proteins in ways we haven’t modelled: future research directions!

We point to lots specific hypotheses, data and knowledge gaps, see Dataset EV1. We’re not pursuing any of them.

@flargh Team #GoForIt